Research shows the important role of gut bacteria in preventing and treating type 1 diabetes

by Barbara Hewitt on September 5, 2017

Scientists have discovered new evidence of the importance of gut bacteria in terms of averting and treating conditions such as type 1 diabetes.

It is already known, namely that certain common variants of HLA/MHC genes protect against a range of autoimmune diseases, particularly type 1 diabetes. Yet how these genes and the tiny cell proteins they regulate yield their immune modulating effects has remained shrouded in mystery.

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Now, a study in mice led by scientists at Harvard Medical School reveals that at least one of these genes has a protective influence that is powerfully shaped by the trillions of intestinal bacteria collectively known as the gut microbiota.

In the study, researchers worked with mice bred to spontaneously develop diabetes, the classic animal model for studying the disease. However, this particular group was also bred to carry a protective gene variant shown in earlier studies to ward off type 1 diabetes despite the animals’ heavy predisposition to the disease.

When treated with antibiotics in the first six weeks of life, mice went on to develop pancreatic inflammation, a precursor to type 1 diabetes, despite carrying the guardian gene. Treatment with antibiotics later in life, between six and 10 weeks after birth, did not lead to loss of protection against diabetes.

The observation suggests a period during which the new born gut is seeded by various germs, the researchers say. Interfering with that process by administering antibiotics appears to disrupt the balance of the gut microbiota, which in turn leads to loss of genetic protection, the researchers said.

The new findings demonstrate that gut bacteria are potent catalysts of autoimmunity and pancreatic cell function and that perturbations in the gut microbiota can precipitate diabetes. The results also open up avenues for immune modulating therapies targeted at maintaining the delicate bacterial balance of the gut microbiota.

‘We believe that our results not only offer a clue into a longstanding mystery but also raise the possibility that substances or environmental influences that alter the intestinal balance can modulate the effects of a powerfully protective gene and shape disease risk,’ said Diane Mathis, who led the study together with Christophe Benoist, both professors in the Department of Microbiology and Immunobiology at Harvard Medical School.

The researchers point out that there are important physiological differences between mice and humans and emphasize that further studies are needed to elucidate precisely how gut bacteria affect gene activity and the risk for an autoimmune attack on the pancreas.

However, the scientists say their results highlight the role of the gut in proper immune function and point to the existence of a critical window in the proper development of the gut microbiome, a time during which the intestines get populated with a variety of bacteria.

‘Our findings need to be borne out in further experiments. However, our results powerfully illustrate the notion that early antibiotic exposure can modulate disease risk and that avoiding or at least minimizing antibiotic treatment in infants and pregnant women during critical periods of development may be a good idea,’ Mathis explained.

They also found that mice whose protective gene was passed on by the father went on to develop diabetes. Mice that inherited a copy of the guardian gene from their mother, however, were resistant to diabetes. The observation highlights the critical protective role of exposing a new born to the mother’s microbiota, which is passed on during birth.

Mice whose mothers had been given antibiotics in the 10 days before giving birth lost their genetic protection, the researchers found, and went on to develop pancreatic inflammation. Mice born with the protective gene but raised in sterile cages and deprived of bacterial exposure during early development never acquired gut microbial balance and disease protection. These animals developed severe pancreatic inflammation typically seen in diabetic mice.

This observation, the researchers point out, further underscores the importance of early environmental exposures to a variety of germs in the proper development of the immune system. The researchers added that the finding is also consistent with the so-called hygiene hypothesis, which posits that the declining number of childhood infections and lack of sufficient germ exposure during early childhood may fuel a person’s lifetime risk for allergic and autoimmune diseases. That link, however, the researchers caution, has yet to be proven.

In a final set of experiments, the team performed faecal transplants in diabetes-prone mice without the protective gene using faecal matter obtained from mice that carried the guardian gene. Following faecal transplantation, the diabetes-prone mice exhibited dramatically reduced pancreatic cell inflammation and did not develop diabetes, a finding that further affirms the role of gut bacteria as a powerful modulator of disease.


The opinions expressed in this article do not necessarily reflect the views of the DiabetesForum.com Community and should not be interpreted as medical advice. Please see your doctor before making any changes to your diabetes management plan.

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